ONKOCET Ltd. has exhibited the devices from its portfolio on the MEDTEC UK exhibition in Birmingham, April 2011 through our partner Medical & Partners.
In the work were used the clinical strains of E.coli, St. aureus, Ps.aeruginosa, P.vulgaris, Candida albicans, which have been the most typical agents of hospital infection. The suspended in the physiological solution daily cultures of microorganisms in the concentration to 106 units/ml on 1 standard loop (0,005 ml) sowed to dense nutrient medium - medium of Ploskirev, yolk- salt agar with the mannitol, blood agar, Endo's medium. The sowing cultures processed by NO -CGF of apparatus "PLASON" during 1, 3 and 5 min from the distance of 6 cm by circular pendular motions. The processed cups incubated in aerothermostat with 37°C for 24 hours, after which was evaluated an increase in the microorganisms. In the check studies the sowings of cultures at the same dose processed in no way.
For checking the assumption about the possibility of NO influence on the factors of the aggression of the microorganisms, which are frequently determined by plasmids, was used the clinical hemolytic strain of E.coli Hly+. After working during 1min was investigated the hemolytic activity of the strain of the directly irradiated culture, also, after its growing for 20 hours in the nourishing bouillon. For obtaining the reliable results three analogous sets of experiments was carried out.
As a result carried out experiment the following data have been obtained. With the exposure into 1 minute is obtained an increase in E.coli, St. aureus, Ps. aeruginosa and Pr. vulgaris, essential rarefactions there was no sowing in comparison with the control. However, an increase in the microflora was absent with exposure 3 and 5 minutes. For refining the minimum bactericidal exposure were used the shorter ranges of working - from 1 to 3 minutes. It was established that with the exposure 1 min 15 it flogged it is noted the rarefaction of sowings, it flogged that considerably increased after working by a duration of 1 min 20, with the working 1 min 30 it flogged increase no longer obtained. During the study the yeast fungi of the kind of Candida their viability remained with all exposures without the visible rarefaction.
During the study the hemolytic activity of the strain of E.coli Hly+ (directly irradiated culture and after its growing for 20 hours in the nourishing bouillon) of the elimination of plasmids it was not observed.
Thus, it is possible to assert that the cells of bacteria, which relate to different, they perish under the action of the exogenous nitrogen oxide with the exposure of 1minut and 30 sec. Reduction in the number of population of microorganisms occurred to more than three orders.